Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13950-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 13950-1-AP
IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug.
IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug.
IHC staining of human stomach cancer using 13950-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13950-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13950-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13950-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).
Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13950-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach cancer using 13950-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13950-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of K-562 using 13950-1-AP
IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug.
IF/ICC Figures
IF Staining of HeLa using 13950-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13950-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
ABCF1 Polyclonal antibody
Catalog Number
13950-1-AP
Citations
3
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
