Filter: AllWBIHCIF

WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.
WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67960-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67960-1-PBS targets ABCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	ABCE1 fusion protein Ag28493 Predict reactive species
Full Name	ATP-binding cassette, sub-family E (OABP), member 1
Calculated Molecular Weight	599 aa, 67 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC016283
Gene Symbol	ABCE1
Gene ID (NCBI)	6059
RRID	AB_2918711
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P61221
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.