ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7E8

Cat no : 67960-1-PBS

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

---

Validation Data Gallery

• 

  WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

---

Product Information

67960-1-PBS targets ABCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCE1 fusion protein Ag28493
• Full Name: ATP-binding cassette, sub-family E (OABP), member 1
• Calculated Molecular Weight: 599 aa, 67 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC016283
• Gene Symbol: ABCE1
• Gene ID (NCBI): 6059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.