ABCC5 Monoclonal antibody

ABCC5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10F11

Cat no : 67955-1-Ig

Synonyms

ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP

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Validation Data Gallery

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  WB analysis using 67955-1-Ig

  Boiled and unboiled HepG2 lysates were subjected to SDS PAGE followed by western blot with 67955-1-Ig (ABCC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67955-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67955-1-Ig (ABCC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, U2OS cells, HEK-293 cells, HeLa cells, K-562 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67955-1-Ig targets ABCC5 in WB, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCC5 fusion protein Ag31674
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 180-200kDa
• GenBank Accession Number: NM_005688
• Gene Symbol: ABCC5
• Gene ID (NCBI): 10057
• RRID: AB_2918706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCC5, also named as MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).