MRP2 Polyclonal antibody

MRP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29261-1-AP

Synonyms

ABC30, ABCC2, CMOAT, CMOAT1, cMRP, DJS, KIAA1010, MRP2

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Validation Data Gallery

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  WB analysis using 29261-1-AP

  37°C incubated A549 cells total extract lysate and 37°C incubated HepG2 cells total extract lysate were subjected to SDS PAGE followed by western blot with 29261-1-AP (MRP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 29261-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29261-1-AP (ABCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 29261-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29261-1-AP (ABCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: 37°C incubated A549 cells, 37°C incubated HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29261-1-AP targets MRP2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRP2 fusion protein Ag30944
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 2
• Calculated Molecular Weight: 1545 aa, 174 kDa
• Observed Molecular Weight: 190-250 kDa
• GenBank Accession Number: BC136419
• Gene Symbol: ABCC2
• Gene ID (NCBI): 1244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)