ABCB4 Polyclonal antibody

ABCB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27726-1-AP

Synonyms

ABC21, ABCB4, GBD1, MDR2, MDR2/3, MDR3, Multidrug resistance protein 3, P glycoprotein 3, PFIC 3, PGY3

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Validation Data Gallery

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  WB analysis using 27726-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27726-1-AP (ABCB4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27726-1-AP targets ABCB4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCB4 fusion protein Ag26840
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 4
• Calculated Molecular Weight: 1286 aa, 142 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC042531
• Gene Symbol: ABCB4
• Gene ID (NCBI): 5244
• RRID: AB_2918128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.