ABCA3 Polyclonal antibody

ABCA3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27450-1-AP

Synonyms

ATP binding cassette 3, ABC-C transporter, ABCA 3, ABC3, ABC C transporter

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Validation Data Gallery

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  WB analysis using 27450-1-AP

  unboiled rat lung tissue were subjected to SDS PAGE followed by western blot with 27450-1-AP (ABCA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: unboiled rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27450-1-AP targets ABCA3 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCA3 fusion protein Ag24749
• Full Name: ATP-binding cassette, sub-family A (ABC1), member 3
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC140895
• Gene Symbol: ABCA3
• Gene ID (NCBI): 21
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCA3 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCA3, which is expressed in alveolar type II pneumocytes and localizes predominantly to the limiting membrane of lamellar bodies, is critical for synthesis of surfactant(PMID: 17267394). Mutation of the ABCA3 gene causes fatal surfactant deficiency in newborns(PMID: 15044640).