ABCA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABCA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26564-1-AP

Synonyms

ABC 1, ABC1, ABCA1, ATP binding cassette 1, CERP, HDLDT1, TGD

---

Validation Data Gallery

• 

  WB analysis using 26564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 26564-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26564-1-AP (ABCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: L02 cells, mouse brain tissue, mouse liver tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

26564-1-AP targets ABCA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCA1 fusion protein Ag24118
• Full Name: ATP-binding cassette, sub-family A (ABC1), member 1
• Calculated Molecular Weight: 2261 aa, 254 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC141816
• Gene Symbol: ABCA1
• Gene ID (NCBI): 19
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Tumour-associated macrophage-derived DOCK7-enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis; Authors - Weiwei Chen ; KD Validated
human,mouse	WB,IHC	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
mouse	WB,IHC	Atherosclerosis; Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B; Authors - Yuanyuan Li