AASDHPPT Polyclonal antibody

AASDHPPT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11244-1-AP

Synonyms

CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT

---

Validation Data Gallery

• 

  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11244-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11244-1-AP (AASDHPPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: human brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11244-1-AP targets AASDHPPT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AASDHPPT fusion protein Ag1763
• Full Name: aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC016728
• Gene Symbol: AASDHPPT
• Gene ID (NCBI): 60496
• RRID: AB_2304862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	bioRxiv; Mitochondrial Phosphopantetheinylation is Required for Oxidative Function; Authors - Pieter R Norden