AAMP Polyclonal antibody

AAMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21220-1-AP

Synonyms

Angio-associated migratory cell protein

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Validation Data Gallery

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  WB analysis using 21220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21220-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21220-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21220-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 21220-1-AP

  Immunofluorescent analysis of MCF-7 cells using 21220-1-AP (AAMP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, human heart tissue, mouse kidney tissue, mouse lung tissue, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

21220-1-AP targets AAMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AAMP fusion protein Ag15655
• Full Name: angio-associated, migratory cell protein
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 52-58 kDa
• GenBank Accession Number: BC039866
• Gene Symbol: AAMP
• Gene ID (NCBI): 14
• RRID: AB_10733642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IF	Exp Ther Med; MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB.; Authors - Xiaogao Wang