AADAT Polyclonal antibody

AADAT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13031-1-AP

Synonyms

2 aminoadipate transaminase, AADAT, aminoadipate aminotransferase, KAT/AadAT, KAT2, KAT-2, KATII, Kynurenine aminotransferase II, kynurenine aminotransferase-2, Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial

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Validation Data Gallery

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  IHC staining of mouse cerebellum using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13031-1-AP (AADAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13031-1-AP (AADAT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13031-1-AP (AADAT antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

13031-1-AP targets AADAT in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AADAT fusion protein Ag3676
• Full Name: aminoadipate aminotransferase
• Calculated Molecular Weight: 429 aa, 48 kDa
• GenBank Accession Number: BC031068
• Gene Symbol: AADAT
• Gene ID (NCBI): 51166
• RRID: AB_2877904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Brain Struct Funct; Astrocytic and neuronal localization of kynurenine aminotransferase-2 in the adult mouse brain.; Authors - Judit Herédi
mouse	IHC	Neurochem Int; Species-specific neuronal localization of kynurenine aminotransferase-2 in the mouse cerebellum.; Authors - Emma Balog
		J Clin Med; Impaired Skeletal Muscle Kynurenine Metabolism in Patients with Chronic Obstructive Pulmonary Disease.; Authors - Harry R Gosker
mouse	IHC,IF	Cell Commun Signal; Helicobacter pylori promotes gastric intestinal metaplasia through activation of IRF3-mediated kynurenine pathway; Authors - Xinhua Liang