ALADIN Polyclonal antibody

• Featured Product
• KD/KO Validated

ALADIN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15127-1-AP

Synonyms

AAA, AAAS, AAASb, ADRACALA, ADRACALIN, ALADIN, GL003

---

Validation Data Gallery

• 

  WB analysis using 15127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15127-1-AP (ALADIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 15127-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15127-1-AP (ALADIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, NIH/3T3 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15127-1-AP targets ALADIN in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse samples.

• Tested Reactivity: human, monkey, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALADIN fusion protein Ag7211
• Full Name: achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC000659
• Gene Symbol: AAAS
• Gene ID (NCBI): 8086
• RRID: AB_2219477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The AAAS gene product is the 60 kDa nuclear pore complex (NPC) protein alacrima-achalasia-adrenal insufficiency neurological disorder (ALADIN). AAAS mRNA and the ALADIN protein are ubiquitously expressed with predominance in the adrenal and central nervous system structures in human and rat. ALADIN, a Tryptophan-Aspartic acid (WD) repeat containing protein, was the first nuclear pore complex protein to be associated with hereditary neurodegenerative disease and the only nuclear pore complex protein to be associated with hereditary adrenal disease. ALADIN's precise function at the nuclear pore complex is unknown. Most naturally occurring AAAS mutations result in mislocalization of the abnormal ALADIN protein (mainly into the cytoplasm), implying that correct NPC targeting is vital for its function. (PMID: 23825130)

Publications

Species	Application	Title
human	WB,IF	Mol Biol Cell; The Nucleoporin ALADIN Regulates Aurora A Localization to Ensure Robust Mitotic Spindle Formation.; Authors - Sara Carvalhal