A4GALT Polyclonal antibody

A4GALT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12392-1-AP

Synonyms

EC:2.4.1.228, Alpha4Gal-T1, Alpha-1,4-N-acetylglucosaminyltransferase, Alpha-1,4-galactosyltransferase, A4GALT1

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 12392-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12392-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12392-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12392-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, K-562 cells, Raji cells
• Positive IHC detected in: human cervical cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12392-1-AP targets A4GALT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: A4GALT fusion protein Ag3069
• Full Name: alpha 1,4-galactosyltransferase
• Calculated Molecular Weight: 353 aa, 41 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC017068
• Gene Symbol: A4GALT
• Gene ID (NCBI): 53947
• RRID: AB_2877853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.