Alpha-2-Macroglobulin Polyclonal antibody

Alpha-2-Macroglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13545-1-AP

Synonyms

A2M, C3 and PZP like alpha 2 macroglobulin domain containing protein 5, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

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Validation Data Gallery

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  WB analysis of human plasma using 13545-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13545-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13545-1-AP

  IP result of anti-Alpha-2-Macroglobulin (IP:13545-1-AP, 4ug; Detection:13545-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13545-1-AP (Alpha-2-Macroglobulin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13545-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (13545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human blood tissue, human brain tissue, HepG2 cells, human plasma tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13545-1-AP targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, plasmodium falciparum
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha-2-Macroglobulin fusion protein Ag4489
• Full Name: alpha-2-macroglobulin
• Calculated Molecular Weight: 1474 aa, 163 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC040071
• Gene Symbol: Alpha 2 Macroglobulin
• Gene ID (NCBI): 2
• RRID: AB_10642837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer's Disease Patients.; Authors - Riikka Lampinen
human	IF	Am J Cancer Res; Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers; Authors - Mateusz Olbromski
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan