5 Lipoxygenase Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

5 Lipoxygenase Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10021-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10021-1-Ig (same clone as 10021-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 10021-1-Ig (same clone as 10021-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10021-1-Ig (same clone as 10021-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

  Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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Product Information

10021-1-PBS targets 5 Lipoxygenase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: arachidonate 5-lipoxygenase
• Calculated Molecular Weight: 674 aa, 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC130332
• Gene Symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).