4EBP1 Recombinant antibody

4EBP1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241055E7

Cat no : 83890-5-RR

Synonyms

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

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Validation Data Gallery

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  WB analysis using 83890-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83890-5-RR (4EBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83890-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HEK-293 using 83890-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83890-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83890-5-RR against Human 4EBP1 were performed. The affinity constant is 0.752 nM.

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Tested Applications

• Positive WB detected in: A549 cells, HL-60 cells, HepG2 cells, HeLa cells, K-562 cells, A431 cells
• Positive FC (Intra) detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83890-5-RR is 4EBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: 4EBP1 fusion protein Ag5056
• Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Calculated Molecular Weight: 118 aa, 12 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC004459
• Gene Symbol: EIF4EBP1
• Gene ID (NCBI): 1978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)