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4-Hydroxynonenal Monoclonal antibody

4-Hydroxynonenal Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

4-hydroxynonenal, chemical compound

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

2C1D10

Cat no : 68538-1-Ig

Synonyms

Cas. 75899-68-2, C9H16O2, 4-Hydroxy-2-nonenal, 4-HNE

Validation Data Gallery

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  • ELISA experiment of 4-Hydroxynonenal using 4-Hydroxynonenal Monoclonal antibody (68538-1-Ig)

    ELISA experiment of 4-Hydroxynonenal using 68538-1-Ig

    Indirect ELISA was performed by coating BSA conjugated 4-Hydroxynonenal (4-HNE) at ~20 ng/well (by 4-HNE amount), followed by blocking with 1% BSA. Serial diluted 4-Hydroxynonenal antibody 68538-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of 4-Hydroxynonenal was mixed in 5 ng/mL primary antibody (bottom X-left Y, red curve).

Tested Applications

Positive ELISA detected in4-Hydroxynonenal

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68538-1-Ig targets 4-Hydroxynonenal in ELISA applications and shows reactivity with 4-hydroxynonenal, chemical compound samples.

Tested Reactivity 4-hydroxynonenal, chemical compound
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTG
Full Name 4-Hydroxynonenal
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

4-Hydroxynonenal is a uremic toxin. Uremic toxins can be subdivided into three major groups based upon their chemical and physical characteristics: 1) small, water-soluble, non-protein-bound compounds, such as urea; 2) small, lipid-soluble and/or protein-bound compounds, such as the phenols and 3) larger so-called middle-molecules, such as beta2-microglobulin. 4-Hydroxynonenal (4-HNE) is a major aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. 4-HNE has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. 4-HNE has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. In addition, it irreversibly inhibits Na+-K+-ATPase activity.