14-3-3 Epsilon Polyclonal antibody

• Featured Product
• KD/KO Validated

14-3-3 Epsilon Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 11648-2-AP

Synonyms

14-3-3E, YWHAE, 14-3-3epsilon, 14-3-3 protein epsilon

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Validation Data Gallery

• 

  WB analysis of A375 using 11648-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11648-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 11648-2-AP

  IP result of anti-14-3-3 Epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

• 

  IHC staining of human colon using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11648-2-AP (14-3-3 epsilon antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 11648-2-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: human colon tissue, human gliomas tissue, human lung cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IF: See 5 publications below
• IP: See 3 publications below

Product Information

11648-2-AP targets 14-3-3 Epsilon in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: 14-3-3 Epsilon fusion protein Ag2247
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Calculated Molecular Weight: 255 aa, 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC000179
• Gene Symbol: 14-3-3E
• Gene ID (NCBI): 7531
• RRID: AB_2217787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.

Publications

Species	Application	Title
human	WB,IF	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu
human	IP	Cell Host Microbe; Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.; Authors - William Riedl
mouse	WB	EMBO J; AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.; Authors - Xin Zheng
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
mouse	WB	Neurobiol Dis; Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models; Authors - Rodrigo Vinueza-Gavilanes

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