Na 1-Methyladenosine Monoclonal antibody, PBS Only

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  Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose. This data was developed using the same antibody clone with 68636-1-PBS in a different storage buffer formulation.

1-Methyladenosine Monoclonal Antibody for Dot Blot, Indirect ELISA

Cat No. 68636-1-PBS

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound

Applications

Dot Blot, Indirect ELISA

Synonyms

N1-methyladenosine, m1A

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Product Information

68636-1-PBS targets 1-Methyladenosine in Dot Blot, Indirect ELISA applications and shows reactivity with chemical compound samples.

• Tested Reactivity: chemical compound
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTG
• Full Name: 1-Methyladenosine
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf