β-Nicotinamide Mononucleotide Monoclonal antibody

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  ELISA experiment of β-Nicotinamide Mononucleotide using 68638-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to β-Nicotinamide Mononucleotide (NMN). Indirect ELISA was performed by coating BSA conjugated β-Nicotinamide Mononucleotide (NMN) at ~10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of β-Nicotinamide Mononucleotide (NMN) were mixed in primary antibody (fixed at 10 ng/mL).

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  ELISA experiment of urine using 68638-1-Ig

  BSA conjugated β-Nicotinamide Mononucleotide (NMN) was coated in 96 well plate at ~10 ng/well (by NMN amount), followed by blocking with 1% BSA. Different concentrations of NMN standard as well as diluted urine from NMN orally administrated mouse were mixed with 10 ng/mL β-Nicotinamide Mononucleotide antibody 68638-1-Ig respectively. Urine NMN was calculated based on standard curve.

β-Nicotinamide Mononucleotide Monoclonal Antibody for ELISA

Cat No. 68638-1-Ig

Clone No.3A1F11

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound

Applications

ELISA

Synonyms

β-NMN, β NMN, NMN

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Tested Applications

• Positive ELISA detected in: β-Nicotinamide Mononucleotide, urine

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68638-1-Ig targets β-Nicotinamide Mononucleotide in ELISA applications and shows reactivity with chemical compound samples.

• Tested Reactivity: chemical compound
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTG
• Full Name: β-Nicotinamide Mononucleotide
• RRID: AB_3085327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicotinamide mononucleotide ("NMN" and "β-NMN") is a nucleotide derived from ribose, nicotinamide, nicotinamide riboside and niacin. In humans, several enzymes use NMN to generate nicotinamide adenine dinucleotide (NADH). In mice, it has been proposed that NMN is absorbed via the small intestine within 10 minutes of oral uptake and converted to nicotinamide adenine dinucleotide (NAD+) through the Slc12a8 transporter. However, this observation has been challenged, and the matter remains unsettled. Maintain cell quality and reduce cell aging with β-nicotinamide mononucleotide (β-NMN), an intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD+) (Bogan & Brenner). β-NMN is a product of the nicotinamide phosphoribosyltransferase (NAMPT) reaction and is converted to NAD+ by nicotinamide-nucleotide adenylyltransferase.