BRAF Monoclonal Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Mouse / IgG2b
Detection: Mouse / IgG1
Reactivity
Capture: human
Detection: human
Applications
Cytometric bead array
Range
Cytometric Bead Array: 0.391-100 ng/mL
Validation Data Gallery
Tested Applications
Cytometric bead array
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP50272-2 targets BRAF in immunoassays as a matched antibody pair. Validated in Cytometric bead array.
Range: 0.391-100 ng/mL (Cytometric Bead Array);
Capture antibody: BRAF Monoclonal antibody, PBS Only (Capture) 68875-1-PBS (1F5E10). 100 μg. Concentration 1 mgl/ml.
Detection antibody: BRAF Monoclonal antibody, PBS Only (Detector) 68875-3-PBS (3F10H7). 100 μg. Concentration 1 mgl/ml.
Alternative BRAF matched antibody pairs: MP50272-1, MP50272-3
Matched pair protein standard: Ag28574
Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 68875-1-PBS |
| Tested Reactivity | human |
| Immunogen | BRAF fusion protein Ag28574 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-raf murine sarcoma viral oncogene homolog B1 |
| Synonyms | Proto-oncogene B-Raf, p94, EC:2.7.11.1, BRAF1, B RAF1 |
| GenBank accession number | BC101757 |
| Gene symbol | BRAF |
| Uniprot | P15056 |
| Gene ID (NCBI) | 673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 68875-3-PBS |
| Tested Reactivity | human |
| Immunogen | BRAF fusion protein Ag28574 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-raf murine sarcoma viral oncogene homolog B1 |
| Synonyms | |
| GenBank accession number | BC101757 |
| Gene symbol | BRAF |
| Uniprot | P15056 |
| Gene ID (NCBI) | 673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G Magarose purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |