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NGF Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG1

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 3.9-250 pg/mL

Cat No : MP50038-1

Print datasheet

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50038-1, NGF Monoclonal Matched Antibody Pair - PBS only. 68661-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1222. 68661-2-PBS was biotinylated as the detection antibody. Range: 3.9-250 pg/mL.

    Sandwich ELISA standard curve of MP50038-1

    Sandwich ELISA standard curve of MP50038-1, NGF Monoclonal Matched Antibody Pair - PBS only. 68661-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1222. 68661-2-PBS was biotinylated as the detection antibody. Range: 3.9-250 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50038-1 targets NGF in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 3.9-250 pg/mL (Sandwich ELISA);

Capture antibody: beta NGF Monoclonal antibody, PBS Only (Capture) 68661-1-PBS (5F2E4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: beta NGF Monoclonal antibody, PBS Only (Detector) 68661-2-PBS (1B2F6). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: HZ-1222

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68661-1-PBS
Tested Reactivity Human
Immunogen beta NGF fusion protein HZ-1222
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=2.69 x 10-8M
KOff=2.17 x 10-4M
KOn=8.06 x 103M
Full Name nerve growth factor (beta polypeptide)
Synonyms Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF
Gene symbol NGF
Gene ID (NCBI) 4803
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68661-2-PBS
Tested Reactivity Human
Immunogen beta NGF fusion protein HZ-1222
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=3.37 x 10-8M
KOff=2.39 x 10-4M
KOn=7.10 x 103M
Full Name nerve growth factor (beta polypeptide)
Synonyms Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF
Gene symbol NGF
Gene ID (NCBI) 4803
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.