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IL-28A Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG1

Reactivity

Capture: human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP50008-1

Print datasheet

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50008-1, IL-28A Monoclonal Matched Antibody Pair - PBS only. 60270-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1235. 68669-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP50008-1

    Sandwich ELISA standard curve of MP50008-1, IL-28A Monoclonal Matched Antibody Pair - PBS only. 60270-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1235. 68669-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50008-1 targets IL-28A in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: IL-28A Monoclonal antibody, PBS Only (Capture) 60270-1-PBS (3B4A1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL-28A Monoclonal antibody, PBS Only (Detector) 68669-1-PBS (1A5C4). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: HZ-1235

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 60270-1-PBS
Tested Reactivity human
Immunogen IL-28A fusion protein Ag18525
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.77 x 109M
KOff=4.10 x 10-5M
KOn=2.31 x 104M
Full Name interleukin 28A (interferon, lambda 2)
Synonyms Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
GenBank accession numberBC113583
Gene symbol IL-28A
UniprotQ8IZJ0
Gene ID (NCBI) 282616
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68669-1-PBS
Tested Reactivity Human
Immunogen IL-28A fusion protein HZ-1235
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=5.94 x 10-9M
KOff=3.48 x 10-4M
KOn=5.86 x 104M
Full Name interleukin 28A (interferon, lambda 2)
Synonyms Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
Gene symbol IL-28A
Gene ID (NCBI) 282616
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.