• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ACVRL1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.781-50 ng/mL

Cat No : MP01306-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01306-2, Human ACVRL1 Recombinant Matched Antibody Pair - PBS only. 84421-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2132. 84421-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

    Sandwich ELISA standard curve of MP01306-2

    Sandwich ELISA standard curve of MP01306-2, Human ACVRL1 Recombinant Matched Antibody Pair - PBS only. 84421-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2132. 84421-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • The mean ACVRL1 concentration was determined to be 34.48 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load, 1108.74 ng/mL in HL-60 cell extract based on a 3.00 mg/mL extract load and 176.59 ng/mL in U-937 cell extract based on a 1.40 mg/mL extract load.

    Sample test of MP01306-2

    The mean ACVRL1 concentration was determined to be 34.48 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load, 1108.74 ng/mL in HL-60 cell extract based on a 3.00 mg/mL extract load and 176.59 ng/mL in U-937 cell extract based on a 1.40 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01306-2 targets ACVRL1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.781-50 ng/mL (Sandwich ELISA);

Capture antibody: ACVRL1 Recombinant antibody, PBS Only (Capture/Detector) 84421-1-PBS (241686B9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ACVRL1 Recombinant antibody, PBS Only (Capture/Detector) 84421-2-PBS (241686D9). 100 μg. Concentration 1 mgl/ml.

Alternative ACVRL1 matched antibody pairs: MP01306-1

Matched pair protein standard: Eg2132

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84421-1-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor type II-like 1
Synonyms ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1
GenBank accession numberBC042637
Gene symbol ACVRL1
UniprotP37023
Gene ID (NCBI) 94
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84421-2-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor type II-like 1
Synonyms ALK1, ALK 1, ACVRLK1, Activin receptor-like kinase 1, Activin receptor like kinase 1
GenBank accession numberBC042637
Gene symbol ACVRL1
UniprotP37023
Gene ID (NCBI) 94
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.