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CD97 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP01158-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01158-4, Human CD97 Recombinant Matched Antibody Pair - PBS only. 84255-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02330. 84255-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

    Sandwich ELISA standard curve of MP01158-4

    Sandwich ELISA standard curve of MP01158-4, Human CD97 Recombinant Matched Antibody Pair - PBS only. 84255-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02330. 84255-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Serum of six mice was measured. The CD97 concentration of detected samples was determined to be 18.14 ng/mL with a range of 15.16-23.41 ng/mL.

    Sample test of MP01158-4

    Serum of six mice was measured. The CD97 concentration of detected samples was determined to be 18.14 ng/mL with a range of 15.16-23.41 ng/mL.

  • The mean CD97 concentration was determined to be 11.39 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load and 16.28 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load.

    Sample test of MP01158-4

    The mean CD97 concentration was determined to be 11.39 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load and 16.28 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01158-4 targets CD97 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: CD97 Recombinant antibody, PBS Only (Capture) 84255-7-PBS (241334H1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD97 Recombinant antibody, PBS Only (Detector) 84255-1-PBS (241334A12). 100 μg. Concentration 1 mgl/ml.

Alternative CD97 matched antibody pairs: MP01158-1, MP01158-2, MP01158-3

Matched pair protein standard: RP02330

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84255-7-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD97 molecule
Synonyms
GenBank accession numberBC026690
Gene symbol CD97
UniprotP48960
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84255-1-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD97 molecule
Synonyms CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5
GenBank accession numberBC026690
Gene symbol CD97
UniprotP48960
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.