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CD8b Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.078-10 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP01133-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01133-1, Human CD8b Recombinant Matched Antibody Pair - PBS only. 84240-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02351. 84240-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP01133-1

    Sandwich ELISA standard curve of MP01133-1, Human CD8b Recombinant Matched Antibody Pair - PBS only. 84240-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02351. 84240-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Serum of six individual healthy human donors was measured. The CD8b concentration of detected samples was determined to be 204 pg/mL with a range of 73-480 pg/mL.

    Sample test of MP01133-1

    Serum of six individual healthy human donors was measured. The CD8b concentration of detected samples was determined to be 204 pg/mL with a range of 73-480 pg/mL.

  • Human peripheral blood mononuclear cells (PBMCs), with HeLa and HEK-293 as negative controls, were cultured for 5 days. The mean concentration of CD8b was measured 99.4 pg/mL in the PBMC supernatant, undetectable in HeLa and HEK-293 supernatants.

    Sample test of MP01133-1

    Human peripheral blood mononuclear cells (PBMCs), with HeLa and HEK-293 as negative controls, were cultured for 5 days. The mean concentration of CD8b was measured 99.4 pg/mL in the PBMC supernatant, undetectable in HeLa and HEK-293 supernatants.

  • Cytometric bead array standard curve of MP01133-1, CD8B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84240-3-PBS. Detection antibody: 84240-1-PBS. Standard: RP02351. Range: 0.078-10 ng/mL.

    Cytometric bead array standard curve of MP01133-1

    Cytometric bead array standard curve of MP01133-1, CD8B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84240-3-PBS. Detection antibody: 84240-1-PBS. Standard: RP02351. Range: 0.078-10 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01133-1 targets CD8b in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.078-10 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: CD8B Recombinant antibody, PBS Only (Capture) 84240-3-PBS (241327C1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD8B Recombinant antibody, PBS Only (Detector) 84240-1-PBS (241326C9). 100 μg. Concentration 1 mgl/ml.

Alternative CD8b matched antibody pairs: MP01133-2

Matched pair protein standard: RP02351

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84240-3-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD8b molecule
Synonyms CD8, 241327C1, Ly3, Leu2, CD8B1
GenBank accession numberBC100913
Gene symbol CD8B
UniprotP10966
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84240-1-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD8b molecule
Synonyms CD8, 241326C9, Ly3, Leu2, CD8B1
GenBank accession numberBC100913
Gene symbol CD8B
UniprotP10966
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.