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NFKB2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 12.7-813 pg/mL

Cat No : MP00950-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00950-1, Human NFKB2 Recombinant Matched Antibody Pair - PBS only. 84022-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0662. 84022-2-PBS was HRP conjugated as the detection antibody. Range: 12.7-813 pg/mL

    Sandwich ELISA standard curve of MP00950-1

    Sandwich ELISA standard curve of MP00950-1, Human NFKB2 Recombinant Matched Antibody Pair - PBS only. 84022-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0662. 84022-2-PBS was HRP conjugated as the detection antibody. Range: 12.7-813 pg/mL

  • The mean NFKB2 concentration was determined to be 1,125.5 pg/mL in Jurkat cell extract based on a 2.3 mg/mL extract load, 2,530.2 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 1,165.1 pg/mL in HEK-293 cell extract based on a 2.0 mg/mL extract load.

    Sample test of MP00950-1

    The mean NFKB2 concentration was determined to be 1,125.5 pg/mL in Jurkat cell extract based on a 2.3 mg/mL extract load, 2,530.2 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 1,165.1 pg/mL in HEK-293 cell extract based on a 2.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP00950-1, NFKB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84022-4-PBS. Detection antibody: 84022-2-PBS. Standard: Ag0662. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP00950-1

    Cytometric bead array standard curve of MP00950-1, NFKB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84022-4-PBS. Detection antibody: 84022-2-PBS. Standard: Ag0662. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00950-1 targets NFKB2 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 12.7-813 pg/mL (Sandwich ELISA);

Capture antibody: NFKB2,p100 Recombinant antibody, PBS Only (Capture/Detector) 84022-4-PBS (241126F8). 100 μg. Concentration 1 mgl/ml.

Detection antibody: NFKB2,p100 Recombinant antibody, PBS Only (Detector) 84022-2-PBS (241126D8). 100 μg. Concentration 1 mgl/ml.

Alternative NFKB2 matched antibody pairs: MP00950-2, MP00950-3

Matched pair protein standard: Ag0662

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84022-4-PBS
Tested Reactivity human
Immunogen NFKB2,p100 fusion protein Ag0662
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2
GenBank accession numberBC002844
Gene symbol NFKB2
UniprotQ00653
Gene ID (NCBI) 4791
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84022-2-PBS
Tested Reactivity human
Immunogen NFKB2,p100 fusion protein Ag0662
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2
GenBank accession numberBC002844
Gene symbol NFKB2
UniprotQ00653
Gene ID (NCBI) 4791
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.