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Conjugate
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Histone H1.0 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse, rat

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 0.937-60 ng/mL

Cat No : MP00895-1

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Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00895-1, Human Histone H1.0 Recombinant Matched Antibody Pair - PBS only. 83993-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9982. 83993-1-PBS was HRP conjugated as the detection antibody. Range: 0.937-60 ng/mL

    Sandwich ELISA standard curve of MP00895-1

    Sandwich ELISA standard curve of MP00895-1, Human Histone H1.0 Recombinant Matched Antibody Pair - PBS only. 83993-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9982. 83993-1-PBS was HRP conjugated as the detection antibody. Range: 0.937-60 ng/mL

  • Hela cell lysate was measured. The human Histone H1.0 concentration of detected samples was determined to be 385.31 ng/mL (based on a 2.0 mg/mL extract load).

    Sample test of MP00895-1

    Hela cell lysate was measured. The human Histone H1.0 concentration of detected samples was determined to be 385.31 ng/mL (based on a 2.0 mg/mL extract load).

  • Cytometric bead array standard curve of MP00895-1, Histone H1.0 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83993-3-PBS. Detection antibody: 83993-1-PBS. Standard: Ag9982. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP00895-1

    Cytometric bead array standard curve of MP00895-1, Histone H1.0 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83993-3-PBS. Detection antibody: 83993-1-PBS. Standard: Ag9982. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00895-1 targets Histone H1.0 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 0.937-60 ng/mL (Sandwich ELISA);

Capture antibody: Histone H1.0 Recombinant antibody, PBS Only (Capture) 83993-3-PBS (241147F9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Histone H1.0 Recombinant antibody, PBS Only (Detector) 83993-1-PBS (241147D2). 100 μg. Concentration 1 mgl/ml.

Alternative Histone H1.0 matched antibody pairs: MP00895-2, MP00895-3

Matched pair protein standard: Ag9982

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83993-3-PBS
Tested Reactivity human
Immunogen Histone H1.0 fusion protein Ag9982
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name H1 histone family, member 0
Synonyms H1F0, H1FV, H1-0, H10, H1 histone family, member 0
GenBank accession numberBC000145
Gene symbol Histone H1.0
UniprotP07305
Gene ID (NCBI) 3005
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83993-1-PBS
Tested Reactivity human, mouse, rat
Immunogen Histone H1.0 fusion protein Ag9982
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name H1 histone family, member 0
Synonyms H1F0, H1FV, H1-0, H10, H1 histone family, member 0
GenBank accession numberBC000145
Gene symbol Histone H1.0
UniprotP07305
Gene ID (NCBI) 3005
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.