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PDK4 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.47-30 ng/mL

Cat No : MP00556-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00556-3, Human PDK4 Recombinant Matched Antibody Pair - PBS only. 83583-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3629. 83583-1-PBS was HRP conjugated as the detection antibody. Range: 0.47-30 ng/mL

    Sandwich ELISA standard curve of MP00556-3

    Sandwich ELISA standard curve of MP00556-3, Human PDK4 Recombinant Matched Antibody Pair - PBS only. 83583-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3629. 83583-1-PBS was HRP conjugated as the detection antibody. Range: 0.47-30 ng/mL

  • Dissect the rat heart tissue and wash briefly with chilled 1X PBS to remove any blood if necessary, cut the tissue into smaller pieces whilst keeping it on ice. Transfer the tissue to a homogenizer and add Extraction Reagent with protease inhibitor. In general, add 500 μL Extraction Reagent for approximately every 10 mg of tissue. Homogenize thoroughly and keep the sample on ice for 30 min. Sonicate the sample and centrifuge at 10,000 x g, then transfer the supernatant to assay. PDK4 in rat heart tissue mesured 38.41 ng/ml total protein.

    Sample test of MP00556-3

    Dissect the rat heart tissue and wash briefly with chilled 1X PBS to remove any blood if necessary, cut the tissue into smaller pieces whilst keeping it on ice. Transfer the tissue to a homogenizer and add Extraction Reagent with protease inhibitor. In general, add 500 μL Extraction Reagent for approximately every 10 mg of tissue. Homogenize thoroughly and keep the sample on ice for 30 min. Sonicate the sample and centrifuge at 10,000 x g, then transfer the supernatant to assay. PDK4 in rat heart tissue mesured 38.41 ng/ml total protein.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00556-3 targets PDK4 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.47-30 ng/mL (Sandwich ELISA);

Capture antibody: PDK4 Recombinant antibody, PBS Only (Capture/Detector) 83583-3-PBS (240551F1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: PDK4 Recombinant antibody, PBS Only (Detector) 83583-1-PBS (240551E4). 100 μg. Concentration 1 mgl/ml.

Alternative PDK4 matched antibody pairs: MP00556-1, MP00556-2

Matched pair protein standard: Ag3629

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83583-3-PBS
Tested Reactivity human
Immunogen PDK4 fusion protein Ag3629
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pyruvate dehydrogenase kinase, isozyme 4
Synonyms PDHK4, EC:2.7.11.2, 240551F1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
GenBank accession numberBC040239
Gene symbol PDK4
UniprotQ16654
Gene ID (NCBI) 5166
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83583-1-PBS
Tested Reactivity human, mouse
Immunogen PDK4 fusion protein Ag3629
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pyruvate dehydrogenase kinase, isozyme 4
Synonyms PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
GenBank accession numberBC040239
Gene symbol PDK4
UniprotQ16654
Gene ID (NCBI) 5166
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.