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MKNK2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-10 ng/mL

Cat No : MP00216-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00216-4, Human MKNK2 Recombinant Matched Antibody Pair - PBS only. 83235-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10546. 83235-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-10 ng/mL.

    Sandwich ELISA standard curve of MP00216-4

    Sandwich ELISA standard curve of MP00216-4, Human MKNK2 Recombinant Matched Antibody Pair - PBS only. 83235-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10546. 83235-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-10 ng/mL.

  • The mean MKNK2 concentration was determined to be 13.14 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

    Sample test of MP00216-4

    The mean MKNK2 concentration was determined to be 13.14 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00216-4 targets MKNK2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-10 ng/mL (Sandwich ELISA);

Capture antibody: MKNK2 Recombinant antibody, PBS Only (Capture) 83235-5-PBS (240045E9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MKNK2 Recombinant antibody, PBS Only (Capture/Detector) 83235-4-PBS (240045D11). 100 μg. Concentration 1 mgl/ml.

Alternative MKNK2 matched antibody pairs: MP00216-1, MP00216-2, MP00216-3

Matched pair protein standard: Ag10546

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83235-5-PBS
Tested Reactivity human
Immunogen MKNK2 fusion protein Ag10546
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAP kinase interacting serine/threonine kinase 2
Synonyms
GenBank accession numberBC018345
Gene symbol MKNK2
UniprotQ9HBH9
Gene ID (NCBI) 2872
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83235-4-PBS
Tested Reactivity human
Immunogen MKNK2 fusion protein Ag10546
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name MAP kinase interacting serine/threonine kinase 2
Synonyms MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1
GenBank accession numberBC018345
Gene symbol MKNK2
UniprotQ9HBH9
Gene ID (NCBI) 2872
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.