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OGT Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-20 ng/mL

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00167-1

Print datasheet

Synonyms

FLJ23071, HRNT1, O GLCNAC, OGT

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00167-1, Human OGT Recombinant Matched Antibody Pair - PBS only. 83113-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2160. 83113-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

    Sandwich ELISA standard curve of MP00167-1

    Sandwich ELISA standard curve of MP00167-1, Human OGT Recombinant Matched Antibody Pair - PBS only. 83113-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2160. 83113-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • The mean OGT concentration was determined to be 2.29 ng/mL in PC-3 cell extract based on a 2.00 mg/mL extract load.

    Sample test of MP00167-1

    The mean OGT concentration was determined to be 2.29 ng/mL in PC-3 cell extract based on a 2.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP00167-1, OGT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83113-3-PBS. Detection antibody: 83113-2-PBS. Standard: Ag2160. Range: 0.625-20 ng/mL.

    Cytometric bead array standard curve of MP00167-1

    Cytometric bead array standard curve of MP00167-1, OGT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83113-3-PBS. Detection antibody: 83113-2-PBS. Standard: Ag2160. Range: 0.625-20 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00167-1 targets OGT in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-20 ng/mL (Cytometric Bead Array); 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: OGT Recombinant antibody, PBS Only (Capture) 83113-3-PBS (230385F11). 100 μg. Concentration 1 mgl/ml.

Detection antibody: OGT Recombinant antibody, PBS Only (Detector) 83113-2-PBS (230385C3). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Ag2160

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83113-3-PBS
Tested Reactivity human
Immunogen OGT fusion protein Ag2160
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255
GenBank accession numberBC014434
Gene symbol OGT
UniprotO15294
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83113-2-PBS
Tested Reactivity human
Immunogen OGT fusion protein Ag2160
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255
GenBank accession numberBC014434
Gene symbol OGT
UniprotO15294
Gene ID (NCBI) 8473
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.