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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 beta protein

Activity(Primary)

Inquire

Species Reactivity

human

Purity

> 95%

Cat no : HZ-1321

Synonyms

Interleukin-12 subunit beta (IL-12B) Cytotoxic lymphocyte maturation factor (CLMF p40), IL-12 subunit p40, NK cell stimulatory factor chain 2 (NKSF2)

Validation Data Gallery

  • Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Technical Specifications

GeneID 3593
Species Human
Expression HEK293
Activity(Primary) Inquire
Purity > 95%
Endotoxin< 0.1 EU/ug
Accession NumberP29460
Molecular Mass 37-50 kDa reduced, 37 to 47, 65 to 95, and 125 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS pH 7.4
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-12p40, also known as IL-12B, is a subunit of both the IL-12 and IL-23 cytokines. It is not thought to be bioactive on its own, but it has been shown to regulate IL-12 activity in antagonistic manner by competitively binding with the IL-12 receptor. IL-12p40 also functions as a chemoattractant molecule for macrophage infiltration into tumor sites. There has additionally been evidence to suggest that IL-12p40 mediates a protective immune response against mycobacterial infection by serving as a necessary component for granuloma formation and promoting Th1 effector cell functions (PMID: 17126601, 10453037, 11739515).

Synonyms

Interleukin-12 subunit beta (IL-12B) Cytotoxic lymphocyte maturation factor (CLMF p40), IL-12 subunit p40, NK cell stimulatory factor chain 2 (NKSF2)