- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IFN gamma protein
Activity(Primary)
0.02-0.14 ng/mL
Specific Activity
minimally 1.00 x 107 IU/mg
Species Reactivity
human,mouse,pig,rat
Purity
>95%
Cat no : HZ-1301
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Validation Data Gallery
Technical Specifications
| GeneID | 3458 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.02-0.14 ng/mL |
| Specific Activity | minimally 1.00 x 107 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P01579 |
| Molecular Mass | 17 and 21 to 32 kDa reduced and non-reduced, homodimer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse,pig,rat |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA). Gently swirl or tap vial to mix. |
Background
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 17, 21-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)
Synonyms
IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma
Publications
| Species | Title |
|---|---|
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | |
EMBO Mol Med Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis. | |
Virulence The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity. | |
Mol Neurobiol Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis | |
Eur J Pharmacol Sappanone A ameliorated imiquimod-induced psoriasis-like dermatitis in BALB/c mice via suppressing Mmp8 expression and IL-17 signaling pathway | |
Cancer Sci CPI-203 improves the efficacy of anti-PD-1 therapy by inhibiting the induced PD-L1 overexpression in liver cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kathirvel (Verified Customer) (05-21-2024) | I have used this cytokine to induce and activate THP-1 derived macrophages and human MDM to check the immune response of macrophages against Mtb. We observed a good immune primed response from the marophages and it helped to get a good results. specially when we tested Mtb mutant strain to subvert host immune response and this cytokine kind of served as a control to study the immune response.
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