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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-4 protein

Activity(Primary)

≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1218

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Validation Data Gallery

  • Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-4 (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 6-30ng/mL.

    Recombinant human FGF-4 (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 6-30ng/mL.

  • The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 2249
Species Human
Expression HEK293
Activity(Primary) ≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP11403
Molecular Mass 17 and 27 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

Fibroblast growth factor-4 (FGF-4) plays an important physiologically role in the self-renewal of human embryonic stem cells (PMID 21990129). It also promotes stem cell proliferation and may aid in differentiation. Xeno-free Recombinant Human FGF-4 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa.

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Publications

SpeciesTitle
mouse

J Cell Commun Signal

Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.

Authors - Yin Cao
View Article