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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-17 (IL-17A) protein

Activity(Primary)

0.24-1.2 ng/mL

Specific Activity

minimally 2.00 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1113

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, IL-17A, interleukin 17A

Validation Data Gallery

  • Purity of recombinant human IL-17A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-17A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-17A (HZ-1113) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 0.24-1.2 ng/mL .

    Recombinant human IL-17A (HZ-1113) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 value range is 0.24-1.2 ng/mL .

Technical Specifications

GeneID 3605
Species Human
Expression HEK293
Activity(Primary) 0.24-1.2 ng/mL
Specific Activityminimally 2.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberQ16552
Molecular Mass 18 and 23 kDa reduced, 29 to 34 kDa non-reduced, homodimer, glycosylated
Formulation 10 mM Tris-HCl pH 7.4+ 250 mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-17 (IL-17A) is a homodimeric glycoprotein with an apparent molecular mass of 30 to 35 kDa. The IL-17 family is comprised of at least 6 proinflammatory cytokines that share a conserved cystine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins that induce local cytokine production and recruit granulocytes to sites of inflammation (PMID: 15485625; 11574464).

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, IL-17A, interleukin 17A

Publications

SpeciesTitle
human

Oncoimmunology

IL-17 induces antitumor immunity by promoting beneficial neutrophil recruitment and activation in esophageal squamous cell carcinoma.

Authors - Chang-Long Chen
View Article
human

J Invest Dermatol

Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.

Authors - Jing Wang
View Article
human

Arthritis Rheumatol

Thymidine phosphorylase regulates the expression of CXCL10 in rheumatoid arthritis fibroblast-like synoviocytes.

Authors - YukoToyoda
View Article
mouse

J Clin Periodontol

Interleukin-17 alleviates erastin-induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p-STAT3

Authors - Jiaqi Bao
View Article
human

Front Immunol

Therapeutic Effects of (5R)-5-Hydroxytriptolide on Fibroblast-Like Synoviocytes in Rheumatoid Arthritis via lncRNA WAKMAR2/miR-4478/E2F1/p53 Axis.

Authors - Xinpeng Zhou
View Article
human

PLoS One

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents.

Authors - KwesiTeye
View Article