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CoraLite® Plus 594-conjugated ZG16 Monoclonal antibody

ZG16 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1A7B9

Cat no : CL594-67389

Synonyms

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite® Plus 594-conjugated ZG16 Monoclonal anti (CL594-67389)

    IF Staining of mouse small intestine using CL594-67389

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9 ) at dilution of 1:100, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), Villin antibody (16488-1-AP, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CoraLite® Plus 594-conjugated ZG16 Monoclonal anti (CL594-67389)

    IF Staining of mouse small intestine using CL594-67389

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9 ) at dilution of 1:100, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), Villin antibody (16488-1-AP, Magenta).

Tested Applications

Positive IF detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67389 targets ZG16 in IF applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZG16 fusion protein Ag11332
Full Name zymogen granule protein 16 homolog (rat)
Calculated Molecular Weight 167 aa, 18 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC029149
Gene Symbol ZG16
Gene ID (NCBI) 653808
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)

Protocols

Product Specific Protocols
IF protocol for CL Plus 594 ZG16 antibody CL594-67389Download protocol
Standard Protocols
Click here to view our Standard Protocols