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Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody

CD80/B7-1 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241371E12

Cat no : 98158-1-RR

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR)

    FC experiment of mouse peritoneal macrophages using 98158-1-RR

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR)

    FC experiment of mouse peritoneal macrophages using 98158-1-RR

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98158-1-RR

    Affinity and Kinetic Characterization of 98158-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98158-1-RR against Mouse CD80/B7-1 were performed. The affinity constant is 1.05 nM.

Tested Applications

Positive FC detected inmouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98158-1-RR is CD80/B7-1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD80 antigen
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_009855.2
Gene Symbol Cd80
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
FC protocol for CD80/B7-1 antibody 98158-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols