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Anti-Human IL-4 Rabbit Recombinant Antibody

IL-4 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240794E11

Cat no : 98138-1-RR

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-4 Rabbit Recombinant Antibody (98138-1-RR)

    FC experiment of human PBMCs using 98138-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-4 Rabbit Recombinant Antibody (98138-1-RR, Clone: 240794E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98138-1-RR

    Affinity and Kinetic Characterization of 98138-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98138-1-RR against Human IL-4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98138-1-RR is IL-4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 4
Calculated Molecular Weight 153 aa, 17 kDa
GenBank Accession NumberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

Protocols

Product Specific Protocols
FC protocol for IL-4 antibody 98138-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols