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Anti-Human CD84 Rabbit Recombinant Antibody

CD84 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241345A3

Cat no : 98108-1-RR

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR)

    FC experiment of human PBMCs using 98108-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR)

    FC experiment of human PBMCs using 98108-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98108-1-RR

    Affinity and Kinetic Characterization of 98108-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98108-1-RR against Human CD84 were performed. The affinity constant is 1.14 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98108-1-RR targets CD84 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD84 molecule
Calculated Molecular Weight 345 aa, 39 kDa
GenBank Accession NumberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).

Protocols

Product Specific Protocols
FC protocol for CD84 antibody 98108-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols