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Anti-Human IL-6 Rabbit Recombinant Antibody

IL-6 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240670D12

Cat no : 98087-1-RR

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR)

    FC experiment of human PBMCs using 98087-1-RR

    1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR)

    FC experiment of human PBMCs using 98087-1-RR

    1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

  • Affinity and Kinetic Characterization of 98087-1-RR

    Affinity and Kinetic Characterization of 98087-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98087-1-RR against Human IL-6 were performed. The affinity constant is 0.322 nM.

Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98087-1-RR is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 6 (interferon, beta 2)
Calculated Molecular Weight 212 aa, 24 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
FC protocol for IL-6 antibody 98087-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols