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Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241297F10

Cat no : 98084-1-RR

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10), or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) (red), or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SELPLG/CD162 Rabbit Recombinant Antibod (98084-1-RR)

    FC experiment of human peripheral blood leukocytes using 98084-1-RR

    1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (98084-1-RR, Clone:241297F10) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98084-1-RR

    Affinity and Kinetic Characterization of 98084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98084-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.699 nM.

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98084-1-RR targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name selectin P ligand
Calculated Molecular Weight 412 aa, 43 kDa
GenBank Accession NumberBC029782
Gene Symbol SELPLG
Gene ID (NCBI) 6404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

Protocols

Product Specific Protocols
FC protocol for SELPLG/CD162 antibody 98084-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols