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Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241283G8

Cat no : 98079-1-RR

Synonyms

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HUVEC cells using Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibod (98079-1-RR)

    FC experiment of HUVEC using 98079-1-RR

    1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8)(red), or 0.25 ug Isotype Control (blue), or 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(black). Cells were not fixed.

  • Flow cytometry (FC) experiment of HUVEC cells using Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibod (98079-1-RR)

    FC experiment of HUVEC using 98079-1-RR

    1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of HUVEC cells using Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibod (98079-1-RR)

    FC experiment of HUVEC using 98079-1-RR

    1x10^6 TNF- α treated HUVEC cells were surface stained with 0.25 ug Anti-Human VCAM-1/CD106 Rabbit Recombinant Antibody (98079-1-RR, Clone:241283G8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98079-1-RR

    Affinity and Kinetic Characterization of 98079-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98079-1-RR against Human VCAM-1/CD106 were performed. The affinity constant is 1.71 nM.

Tested Applications

Positive FC detected inTNF- α treated HUVEC cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98079-1-RR targets VCAM-1/CD106 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name vascular cell adhesion molecule 1
Calculated Molecular Weight81kDa
GenBank Accession NumberNM_001078.4
Gene Symbol VCAM-1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Protocols

Product Specific Protocols
FC protocol for VCAM-1/CD106 antibody 98079-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols