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Anti-Mouse CD200 Rabbit Recombinant Antibody

CD200 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240391A6

Cat no : 98039-1-RR

Synonyms

OX2, Mox2, CD200 antigen, 240391A6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR)

    FC experiment of mouse splenocytes using 98039-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR, Clone: 240391A6) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2) (CL647-65139, Clone: RA3-6B2). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR)

    FC experiment of mouse splenocytes using 98039-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD200 Rabbit Recombinant Antibody (98039-1-RR, Clone: 240391A6) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98039-1-RR

    Affinity and Kinetic Characterization of 98039-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98039-1-RR against Mouse CD200 were performed. The affinity constant is 0.879 nM.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98039-1-RR is CD200 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD200 antigen
Calculated Molecular Weight31 kDa
Gene Symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Protocols

Product Specific Protocols
FC protocol for CD200 antibody 98039-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols