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  • Western Blot (WB) analysis of various lysates using NPTX2 Recombinant antibody (86203-1-RR)

    WB analysis using 86203-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86203-1-RR (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using NPTX2 Recombinant antibody (86203-1-RR)

    WB analysis of SH-SY5Y using 86203-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 86203-1-RR (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NPTX2 Recombinant antibody

NPTX2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86203-1-RR
Clone No.250809A7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86203-1-RR targets NPTX2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NPTX2 fusion protein Ag33808 Predict reactive species
Full Name neuronal pentraxin II
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC009924
Gene Symbol NPTX2
Gene ID (NCBI) 4885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47972
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Protocols

Product Specific Protocols
WB protocol for NPTX2 antibody 86203-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols