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  • Western Blot (WB) analysis of various lysates using PTK6/BRK Recombinant antibody (85352-2-RR)

    WB analysis using 85352-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85352-2-RR (PTK6/BRK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PTK6/BRK Recombinant antibody (85352-2-RR)

    IF Staining of A549 using 85352-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PTK6 antibody (85352-2-RR, Clone: 242647F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PTK6/BRK Recombinant antibody

PTK6/BRK {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85352-2-RR
Clone No.242647F4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PTK6, BRK, 242647F4, Protein-tyrosine kinase 6, Protein tyrosine kinase 6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85352-2-RR targets PTK6/BRK in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name PTK6 protein tyrosine kinase 6
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC035843
Gene Symbol PTK6
Gene ID (NCBI) 5753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13882
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRK, also named as PTK6 and FLJ42088, is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. PTK6 can phosphorylate KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. This antibody is specific to PTK6.

Protocols

Product Specific Protocols
WB protocol for PTK6/BRK antibody 85352-2-RRDownload protocol
IF protocol for PTK6/BRK antibody 85352-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols