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  • Western Blot (WB) analysis of various lysates using NUP214 Recombinant antibody (85161-4-RR)

    WB analysis using 85161-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85161-4-RR (NUP214 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NUP214 Recombinant antibody (85161-4-RR)

    IHC staining of mouse kidney using 85161-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NUP214 Recombinant antibody (85161-4-RR)

    IHC staining of mouse kidney using 85161-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUP214 Recombinant antibody (85161-4-RR)

    IHC staining of mouse testis using 85161-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NUP214 Recombinant antibody (85161-4-RR)

    IHC staining of mouse testis using 85161-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NUP214 Recombinant antibody

NUP214 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85161-4-RR
Clone No.242782H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA0023, CAN, CAIN, 242782H2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HEK-293T cells, MCF-7 cells, A431 cells, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85161-4-RR targets NUP214 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUP214 fusion protein Ag21377 Predict reactive species
Full Name nucleoporin 214kDa
Calculated Molecular Weight 2080 aa, 213 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC105998
Gene Symbol NUP214
Gene ID (NCBI) 8021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35658
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).

Protocols

Product Specific Protocols
WB protocol for NUP214 antibody 85161-4-RRDownload protocol
IHC protocol for NUP214 antibody 85161-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols