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  • Western Blot (WB) analysis of various lysates using NAP1;NCKAP1 Recombinant antibody (85142-2-RR)

    WB analysis using 85142-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85142-2-RR

    Affinity and Kinetic Characterization of 85142-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

NAP1;NCKAP1 Recombinant antibody

NAP1;NCKAP1 {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 85142-2-RR
Clone No.242784C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85142-2-RR targets NAP1;NCKAP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NAP1;NCKAP1 fusion protein Ag2788 Predict reactive species
Full Name NCK-associated protein 1
Calculated Molecular Weight 129 kDa
Observed Molecular Weight 115-125 kDa
GenBank Accession NumberBC015025
Gene Symbol NCKAP1
Gene ID (NCBI) 10787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2A7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

Protocols

Product Specific Protocols
WB protocol for NAP1;NCKAP1 antibody 85142-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols