Filter: AllWBIF/ICCAffinity

WB analysis using 85114-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat thymus using 85114-1-RR
rat thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 85114-1-RR
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of HEL using 85114-1-RR
Immunofluorescent analysis of (4% PFA) fixed HEL cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Jurkat using 85114-1-RR
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 85114-1-RR
Biolayer interferometry (BLl) kinetic assays of 85114-1-RR against Human HCLS1 were performed. The affinity constant is 4.71 nM.

HCLS1 Recombinant antibody

HCLS1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85114-1-RR

Clone No.242825E4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

LckBP1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, CTTNL

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse thymus tissue, rat thymus tissue, Ramos cells, Daudi cells, Raji cells
Positive IF/ICC detected in	HEL cells, Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85114-1-RR targets HCLS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	HCLS1 fusion protein Ag8645 Predict reactive species
Full Name	hematopoietic cell-specific Lyn substrate 1
Calculated Molecular Weight	486 aa, 54 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC016758
Gene Symbol	HCLS1
Gene ID (NCBI)	3059
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P14317
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.