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  • Western Blot (WB) analysis of various lysates using HNRNPR Recombinant antibody (85078-1-RR)

    WB analysis using 85078-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85078-1-RR (HNRNPR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPR Recombinant antibody (85078-1-RR)

    IF Staining of HepG2 using 85078-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPR antibody (85078-1-RR, Clone: 242734C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85078-1-RR

    Affinity and Kinetic Characterization of 85078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85078-1-RR against Human HNRNPR were performed. The affinity constant is 0.545 nM.

HNRNPR Recombinant antibody

HNRNPR {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85078-1-RR
Clone No.242734C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HNRPR, hnRNP R, 242734C8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inrat brain tissue, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, U2OS cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85078-1-RR targets HNRNPR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPR fusion protein Ag30339 Predict reactive species
Full Name heterogeneous nuclear ribonucleoprotein R
Calculated Molecular Weight 71 kDa
Observed Molecular Weight65-82 kDa
GenBank Accession NumberBC001449
Gene Symbol HNRNPR
Gene ID (NCBI) 10236
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)

Protocols

Product Specific Protocols
WB protocol for HNRNPR antibody 85078-1-RRDownload protocol
IF protocol for HNRNPR antibody 85078-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols