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  • Western Blot (WB) analysis of various lysates using TIGAR Recombinant antibody (85052-4-RR)

    WB analysis using 85052-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85052-4-RR (TIGAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85052-4-RR

    Affinity and Kinetic Characterization of 85052-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85052-4-RR against Human TIGAR were performed. The affinity constant is 0.308 nM.

TIGAR Recombinant antibody

TIGAR {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 85052-4-RR
Clone No.242625E7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C12orf5, 242625E7, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HCT 116 cells, Jurkta cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85052-4-RR targets TIGAR in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TIGAR fusion protein Ag17532 Predict reactive species
Full Name chromosome 12 open reading frame 5
Calculated Molecular Weight 270 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012340
Gene Symbol TIGAR
Gene ID (NCBI) 57103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQ88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.

Protocols

Product Specific Protocols
WB protocol for TIGAR antibody 85052-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols