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KIF17 Recombinant antibody

KIF17 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242469G4

Cat no : 85046-1-RR

Synonyms

Kinesin-like protein KIF17, KIF3X, KIF3-related motor protein, KIAA1405, 242469G4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF17 Recombinant antibody (85046-1-RR)

    WB analysis using 85046-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85046-1-RR (KIF17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KIF17 Recombinant antibody (85046-1-RR)

    IHC staining of mouse brain using 85046-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85046-1-RR (KIF17 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85046-1-RR

    Affinity and Kinetic Characterization of 85046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85046-1-RR against Human KIF17 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85046-1-RR targets KIF17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIF17 fusion protein Ag6217
Full Name kinesin family member 17
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC065927
Gene Symbol KIF17
Gene ID (NCBI) 57576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).

Protocols

Product Specific Protocols
WB protocol for KIF17 antibody 85046-1-RRDownload protocol
IHC protocol for KIF17 antibody 85046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols